Thermo Fisher Scientific Calpastatin Monoclonal Antibody (2G11D6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunohistochemistry (Frozen) (IHC (F))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:1,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Bovine, Dog, Human, Pig, Rabbit, Rat

Published species

Bovine, Dog, Fish, Human, Rabbit

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2G11D6

Immunogen

Bovine skeletal muscle calpastatin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calpastatin,uniProtId:P20811-1,ncbiNodeId:9913,antigenRange:1-705,antigenLength:705,antigenImageFileName:MA3-945_Calpastatin_P20811-1_House_mouse.svg,antigenImageFileNamePDP:MA3-945_Calpastatin_P20811-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2259757

Product Specific Information

MA3-945 detects calpastatin from bovine, human, canine, pig, rabbit, and rat tissues and cells. This antibody does not cross-react with calpains or calmodulin.

MA3-945 has been successfully used in Western blot, immunohistochemistry, and immunocytochemical procedures. By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins which have been suggested to be proteins translated from different start sites of the calpastatin gene. Immunofluorescence staining of calpastatin in porcine LLC-PK1 cells with MA3-945 results in diffuse cytoplasmic staining and weak nuclear staining.

The MA3-945 antigen is bovine skeletal muscle calpastatin. This antibody recognizes an epitope found between amino acid residues 295-501 (domain II) of human calpastatin.

Target Information

Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.