Thermo Fisher Scientific PARP1 Monoclonal Antibody (C-2-10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunocytochemistry (ICC/IF)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C-2-10

Immunogen

Purified calf thymus poly (ADP-ribose) polymerase. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PARP1,uniProtId:P18493-1,ncbiNodeId:9913,antigenRange:1-1016,antigenLength:1016,antigenImageFileName:MA3-950_PARP1_P18493-1_House_mouse.svg,antigenImageFileNamePDP:MA3-950_PARP1_P18493-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325523

Product Specific Information

This antibody does not detect PARP from chicken tissues.

By Western blot, this antibody recognizes a 116 kDa protein which corresponds to PARP and the 85 kDa apoptosis-induced cleavage product of prICE and CPP32 from rat thymus extract.

This antibody recognizes an epitope found between amino acids 216-375 in the DNA-binding domain of PARP.

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.