Thermo Fisher Scientific Calpastatin Monoclonal Antibody (1F7E3D10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunocytochemistry (ICC/IF)

1:200

-

Product Specifications

Species Reactivity

Bovine, Human, Pig, Rat

Published species

Bovine, Fish, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1F7E3D10

Immunogen

Purified bovine skeletal muscle 80 kDa subunit of m-Calpastatin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calpastatin,uniProtId:P20811-1,ncbiNodeId:9913,antigenRange:1-705,antigenLength:705,antigenImageFileName:MA3-944_Calpastatin_P20811-1_House_mouse.svg,antigenImageFileNamePDP:MA3-944_Calpastatin_P20811-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2259758

Product Specific Information

MA3-944 detects calpastatin from bovine, human, pig and rat tissues and cells. This antibody does not cross-react with calpains or calmodulin.

MA3-944 has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins from bovine skeletal muscle extract which have been suggested to be proteins translated from different start sites of the calpastatin gene. Immunocytochemical staining of calpastatin in porcine LLC-PK1 cells with MA3-944 results in diffuse cytoplasmic staining.

The MA3-944 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpastatin. This antibody recognizes an epitope between amino acid residues 543-673 (domain IV) of human calpastatin.

Target Information

Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.