Thermo Fisher Scientific FAK Monoclonal Antibody (ZF002), Alexa Fluor 488

Applications and Tested Dilution

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ZF002

Immunogen

Recombinant protein derived from the N-terminal region of the mouse and rat FAK-1 proteins.

Conjugate

Alexa Fluor™ 488

Additional Formats

• Unconjugated
• Alexa Fluor 555
• Alexa Fluor 647
• Request custom conjugation

Excitation / Emission Max

499 / 520 nm
(View spectra below)

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage Buffer

PBS with 1.0 mg/mL BSA

Contains

0.05% sodium azide

Storage Conditions

4°C, store in the dark. Do not freeze.

Shipping Conditions

Ambient (domestic); Dry ice (international)

RRID

AB_2724277

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Product Specific Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and is involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, activating cell migration and invasion.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. It plays a critical role in cell adhesion, spreading, differentiation, and migration. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions—sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, leading to activation of ERK2 MAP kinase. In v-Src-transformed NIH3T3 cells, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion. The Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK at Tyr925. Several transcript variants encoding different isoforms have been identified for the FAK gene.

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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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