Thermo Fisher Scientific Bovine Serum Albumin Monoclonal Antibody (5F9)
소 혈청 알부민(BSA)을 특이적으로 인식하는 단클론 항체로 Western blot, IHC, ELISA, IP 등에 사용 가능. Mouse IgG1 형식으로 제작되었으며 Protein A로 정제된 액상 시약. -20°C 보관 시 안정하며 교차 반응 없음.
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Thermo Fisher Scientific Bovine Serum Albumin Monoclonal Antibody (5F9)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| ELISA | 1:120,000 |
| Immunoprecipitation (IP) | 1:2,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5F9 |
| Immunogen | Anti-Bovine Serum Albumin antibody was produced by repeated immunizations of Balb/c mice with bovine serum albumin. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Reacts specifically with bovine serum albumin. The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross-reactivity detected against other serum proteins by immunoblot. No reactivity detected by ELISA against human serum albumin or ovalbumin.
Target Information
Albumin is a soluble, monomeric protein comprising about half of blood serum protein. It functions primarily as a carrier for steroids, fatty acids, and thyroid hormones, and helps stabilize extracellular fluid volume.
Albumin is a globular, unglycosylated serum protein with a molecular weight of approximately 65,000. It is synthesized in the liver as preproalbumin, which undergoes cleavage to proalbumin and then to secreted albumin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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