Thermo Fisher Scientific Bovine Serum Albumin Monoclonal Antibody (5F9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (IHC)

1:1,000-1:5,000

-

ELISA (ELISA)

1:120,000

-

Immunoprecipitation (IP)

1:2,000-1:10,000

-

Product Specifications

Species Reactivity

Bovine

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5F9

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reacts with bovine serum albumin. The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.

Target Information

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.