Thermo Fisher Scientific Synaptophysin Recombinant Rabbit Monoclonal Antibody (8H2L12)
Synaptophysin 단백질을 검출하기 위한 재조합 토끼 단클론 항체. Western blot 및 면역세포화학(ICC/IF)에 적합. 높은 특이성과 재현성을 제공하며, 배치 간 일관성이 우수함. 단기 4°C, 장기 -20°C 보관 권장.
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8H2L12 |
| Immunogen | Human Synaptophysin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532488 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the larger rabbit immune repertoire
Target Information
Synaptophysin is a calcium-binding integral membrane glycoprotein located in presynaptic vesicles of nearly all neurons. It has four transmembrane domains and forms a complex with dynamin at high calcium concentrations, indicating a role in synaptic vesicle endocytosis.
It also contributes to the regulation of short- and long-term synaptic plasticity and is widely used as a marker for nerve terminals and neuroendocrine tumor differentiation.
Mutations in the synaptophysin gene have been associated with X-linked mental retardation 96.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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