Thermo Fisher Scientific mTOR Recombinant Rabbit Monoclonal Antibody (7H13L18)
Recombinant rabbit monoclonal antibody targeting human mTOR (FRAP1). Validated for WB and ICC/IF. Provides high specificity, sensitivity, and lot-to-lot consistency. Suitable for cancer and cell signaling research. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–3 µg/mL | View 3 publications |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7H13L18 |
| Immunogen | Recombinant protein corresponding to amino acids 1126–1411 of human mTOR |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532486 |
Product Specific Information
- Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits.
- Individual clones are screened to select optimal candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3K/AKT pathway are associated with various cancers. FRAP1 is also the target of rapamycin and its analogues, used as immunosuppressants and cancer therapeutics. Mutations in this gene result in Smith-Kingsmore syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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