Thermo Fisher Scientific IFN gamma Monoclonal Antibody (CC302), PE

Applications

• Flow Cytometry (Flow)
  Tested Dilution: Neat-1:10

Product Specifications

Product Specific Information

Reconstitute with 1 mL of distilled water.
Mouse anti-Bovine IFN gamma; antibody clone CC302 recognizes bovine interferon-gamma, a 143 amino acid cytokine with potent activating, antiviral, and antiproliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence and has a molecular weight of approximately 20 kDa.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and other cytokines.
It is a dimeric glycoprotein consisting of two 146 amino acid subunits, forming a functional homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Its biological activity is species-specific, with human IFN gamma not cross-reactive with mouse IFN gamma.
Functions include:

• Antiviral and tumor antiproliferative activity
• Induction of MHC class I and II
• Macrophage activation
• Enhanced immunoglobulin secretion by B lymphocytes

IFN gamma acts via IFN gamma receptor I and II, activating the JAK-STAT pathway. It shares ~40% sequence homology between human and mouse IFN gamma. Expression is upregulated by IL2, FGF basic, EGF, and downregulated by vitamin D3 or DMN. Mutations in the IFN gamma gene are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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