Thermo Fisher Scientific IFN gamma Monoclonal Antibody (CC330)
소, IFN-γ를 인식하는 단클론 항체(CC330)로 ELISA 및 유세포분석에 사용 가능. 단백질 A로 정제된 비결합형 액상 항체이며, 1 mg/mL 농도. 면역조절 및 사이토카인 연구용으로 적합.
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Applications
- Flow Cytometry (Flow): Assay-dependent
- ELISA: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CC330 |
| Immunogen | Recombinant bovine IFN-gamma |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745422 |
Product Specific Information
Mouse anti-bovine interferon γ antibody, clone CC330 recognizes bovine interferon gamma, a 143 amino acid, approximately 20 kDa immunomodulatory cytokine secreted predominantly by T lymphocytes in response to infection by invading microorganisms.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin, and other cytokines.
It is a dimeric glycoprotein consisting of two 146 amino acid subunits (approx. 45 kDa homodimer). On SDS-PAGE, IFN gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Its biological activity is highly species-specific; human IFN gamma does not cross-react with mouse. Functions include:
- Antiviral activity
- Tumor antiproliferative activity
- Induction of class I and II MHC
- Macrophage activation
- Enhanced immunoglobulin secretion by B lymphocytes
IFN gamma acts synergistically with other cytokines and activates the JAK-STAT pathway via IFN gamma receptor I and II.
It shows no homology with IFN alpha or beta, but human IFN gamma has ~40% sequence homology with mouse IFN gamma.
Expression is upregulated by IL2, FGF basic, EGF, and downregulated by vitamin D3 or DMN.
Mutations in the IFN gamma gene are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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