Thermo Fisher Scientific NFkB p52/p100 Monoclonal Antibody (16B2)
Human NFkB p52/p100 인식 단일클론 항체로 Western blot, IHC, Flow cytometry에 적합. Protein A 정제, 2 mg/mL 농도, PBS/glycerol buffer 포함. Human tonsil 및 placenta 등에서 양성 대조로 사용 가능. 연구용 시약으로 장기보관 시 -20°C 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16B2 |
| Immunogen | Synthetic peptide within Human NFKB2 aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2897726 |
Product Specific Information
Positive controls: Human tonsil tissue, human placenta tissue, A431 cell, Siha cell lysates.
Target Information
The NFkB transcription factor was originally identified as a protein complex consisting of a DNA-binding subunit and an associated protein. The subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a unique protein derived from the amino-terminus of a precursor designated p105. A cDNA encoding an alternative DNA-binding subunit of NFkB has been isolated. It is synthesized and expressed in various cell types and, like p105, undergoes cleavage to generate its NFkB subunit, designated p52 (previously p49). In contrast to p50 derived from p105, p52 acts synergistically with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일명: MA5-37802_NFkB_p52p100_Q00653-1_House_mouse.svg, MA5-37802_NFkB_p52p100_Q00653-1_House_mouse_PDP.jpeg)
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