Thermo Fisher Scientific Desmin Monoclonal Antibody (DE-U-10), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Product Specifications

Species Reactivity

Bovine, Chicken, Cat, Goat, Hamster, Human, Mouse, Sheep, Pig, Rabbit, Rat

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

DE-U-10

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• eFluor 660
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865407

Product Specific Information

Description: The monoclonal antibody DE-U-10 recognizes desmin. Desmin is the protein subunit of class-III intermediate filaments. Desmin is found predominantly in skeletal, cardiac, and smooth muscle. Desmin plays a role in muscle cell development and differentiation, muscle cell architecture and structure, and mitochondrial function. Desmin forms scaffolds around the Z-disk of sarcomeres in muscle cells. Desmin knockout mice exhibit defects in skeletal, smooth, and cardiac muscle and have impaired mitochondrial function. Mutations in Desmin result in conditions such as desmin-related myopathy (DRM), cardiomyopathy dilated type 1I (CMD1I), and neurogenic scapuloperoneal syndrome Kaeser type (Kaeser syndrome).

The DE-U-10 antibody recognizes human, mouse, rat, feline, bovine, goat, hamster, sheep, porcine, rabbit, and chicken desmin. The DE-U-10 antibody will also label tumors derived from muscle tissue, such as leiomyomas and rhabdomyosarcomas.

Applications Reported: This DE-U-10 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This DE-U-10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. This DE-U-10 antibody has been tested by immunocytochemistry of methanol-fixed and permeabilized mouse cells and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.