Thermo Fisher Scientific Thrombospondin-1 Monoclonal Antibody (A6.1), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

1 µg/mL

-

ELISA (ELISA)

Assay-Dependent

-

Immunoprecipitation (IP)

Assay-Dependent

-

Functional Assay (Functional)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

A6.1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572987

Product Specific Information

Description: The A6.1 monoclonal antibody recognizes human, mouse, and rat thrombospondin-1, a member of the thrombospondin family of extracellular calcium-binding proteins. Thrombospondin-1 is a secreted glycoprotein that was first discovered in activated platelets but is also expressed by endothelial cells, adipocytes, fibroblasts, macrophages, monocytes, and smooth muscle cells. Thrombospondin-1 functions by binding members of the extracellular matrix such as fibronectin, integrins, and heparin, and its signaling is mediated through activation of the TGF beta signaling pathway. Thrombospondin-1 has been shown to be involved in angiogenesis, inflammation, osteogenesis, cell proliferation, and synaptogenesis during neural development.

Applications Reported: This A6.1 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, ELISA, and immunocytochemistry.

Applications Tested: This A6.1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 1 µg/mL. This A6.1 antibody has been tested by immunocytochemistry of formaldehyde or methanol fixed and permeabilized human cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.