Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), eBioscience
HLA-DR 단백질을 인식하는 LN3 클론의 마우스 단클론 항체로, 인간 항원제시세포(APC) 분석에 적합합니다. Flow cytometry, IHC 등 다양한 응용에 사용 가능하며, 고순도(>90%)로 정제된 액상 항체입니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1000 | - |
| Immunohistochemistry (IHC) | - | 8 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 5 publications |
| Immunocytochemistry (ICC/IF) | - | 5 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | 32 publications |
| ELISA | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LN3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865529 |
Available Conjugated Formats
- Alexa Fluor 660, 700, Plus 488, Plus 647
- APC, APC-eFluor 780
- Biotin
- Brilliant UV (395–805), Brilliant Violet (421–786)
- eFluor 450, 506
- FITC
- NovaFluor (Blue, Violet, UV, Yellow series)
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright series (436–780)
- Custom conjugation available upon request
Product Specific Information
Description:
The LN3 monoclonal antibody recognizes human MHC class II molecule HLA-DR, expressed on antigen-presenting cells such as B cells, monocytes, macrophages, dendritic cells, and activated T cells. HLA-DR plays a key role in peptide presentation to CD4⁺ T lymphocytes.
Applications Reported:
Suitable for flow cytometry and immunohistochemistry (frozen or paraffin-embedded human tissues).
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.125 µg/test in 100 µL final volume. Optimal titration is advised for specific assays.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB). The DRA gene has 5 exons encoding leader peptide, extracellular domains, and transmembrane/cytoplasmic regions.
DRA acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5, while DRB1 contains polymorphisms that determine peptide-binding specificity.
HLA-DR is mainly expressed on antigen-presenting cells (B cells, monocytes, macrophages, thymic epithelial cells, activated T cells).
MHC class II molecules (DR, DQ, DP) present processed peptides to T-helper cells, initiating immune responses.
HLA/MHC antibodies are valuable tools in immunopeptidomics for neoantigen identification via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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