Thermo Fisher Scientific MHC I Free Chain Monoclonal Antibody (A4), eBioscience

Applications

Immunohistochemistry (IHC)

• Tested Dilution: -
• Publications: View 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-Dependent
• Publications: View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 0.5 µg/test

Immunoprecipitation (IP)

• Tested Dilution: Assay-Dependent

Product Specifications

Additional Formats

• APC Conjugate
• Request custom conjugation

Product Specific Information

Description:
The A4 monoclonal antibody reacts with the class I human leukocyte antigen (HLA) complex in the absence of beta2 microglobulin. There are three class I alpha-chain genes in humans, called HLA-A, -B, and -C. Normally, HLA class I proteins are associated non-covalently with beta2 microglobulin (β2M). The function of the HLA class I complex is to present peptides derived from intracellular pathogens, including viral pathogens, on the cell surface to antigen-specific CD8+ cytotoxic T cells. Class I HLA is expressed by the majority of nucleated cells.

Applications Reported:
This A4 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of paraffin-embedded tissue sections.

Applications Tested:
This A4 antibody has been tested by flow cytometric analysis of 3-day PHA-activated normal human peripheral blood cells. It can be used at ≤0.5 µg per test. A test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL. Cell number should be determined empirically (typically 10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

There are three class I alpha-chain genes in humans, called HLA-A, -B, and -C. Normally, HLA class I proteins are associated non-covalently with β2 microglobulin (β2M). The HLA class I complex presents peptides from intracellular pathogens on the cell surface to CD8+ cytotoxic T cells. Class I HLA is expressed by most nucleated cells.

HLA and MHC antibodies play a crucial role in Immunopeptidomics, facilitating neoantigen identification and characterization via high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.