Thermo Fisher Scientific CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), Biotin, eBioscience

Thermo Fisher Scientific CD357 (AITR/GITR) Monoclonal Antibody (DTA-1), Biotin, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 ng/test
Publications: View 1 publication

Product Specifications

Available Formats

• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The DTA-1 monoclonal antibody reacts with mouse GITR (Glucocorticoid-Induced TNFR family gene, TNFRSF18). In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. Removal of GITR-expressing Treg cells or administration of DTA-1 resulted in organ-specific autoimmune disease.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.5 ng/test (0.0005 µg/test).
A test is defined as the amount of antibody staining a cell sample in 100 µL final volume. Cell number can range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.
Note: Concentration suggested above has been modified from previous lots of biotin-conjugated DTA-1 antibody.

Target Information

Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also called GITR or AITR, is encoded by the TNFRSF18 gene located at 1p36.33. This receptor shows increased expression upon T-cell activation and plays a key role in immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice suggest its involvement in CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.