Thermo Fisher Scientific CD18 Monoclonal Antibody (TS1/18)
인간 CD18 단백질을 인식하는 TS1/18 클론 단일클론 항체로, WB, IHC, ICC/IF, FACS 등 다양한 응용에 사용 가능. 단백질 G 정제, 보존제 무첨가 PBS 용액 형태. 엔도톡신 ≤10 EU/mg으로 검증되어 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:10–1:100 | - |
| Flow Cytometry (Flow) | 0.5 µg/test | 9 publications |
| Neutralization (Neu) | - | 23 publications |
| Functional Assay | - | 2 publications |
| Inhibition Assays (IA) | Assay-dependent | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TS1/18 |
| Immunogen | Human Beta 2 Integrin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_223514 |
Product Specific Information
MA1810 targets CD18 in FACS, IA, and ICC/IF applications and shows reactivity with human samples.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of ≤10 EU/mg.
The immunogen is human Beta 2 Integrin, and MA1810 detects CD18 with a predicted molecular weight of approximately 83 kDa.
Target Information
CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leukocytes.
It forms heterodimers with CD11 molecules (CD11a–d) to constitute leukocyte (β2) integrins:
- αLβ2 (CD11a/CD18, LFA-1)
- αMβ2 (CD11b/CD18, Mac-1, CR3)
- αXβ2 (CD11c/CD18)
- αDβ2 (CD11d/CD18)
These integrins are essential for leukocyte migration and intercellular adhesion.
Deficiency of CD18 leads to leukocyte adhesion deficiency type I (LAD1), and partial reduction is linked to psoriasiform skin disease.
CD18 also mediates leukotoxin-induced cytolysis by Mannheimia haemolytica.
Two transcript variants encoding the CD18 protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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