Thermo Fisher Scientific CD104 Monoclonal Antibody (450-9D)
인간 및 족제비 반응성 CD104 단클론 항체로, 베타4 인테그린을 인식합니다. WB, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, 액상 형태로 제공됩니다. 포름알데히드 고정에 민감하므로 동결 절편은 아세톤 고정 권장.
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Thermo Fisher Scientific CD104 Monoclonal Antibody (450-9D)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | View publication |
| Immunohistochemistry (IHC) | - | View publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:500–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 1:25–1:100 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mustelid |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 450-9D |
| Immunogen | Purified alpha6 beta4 integrin from A431 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_927389 |
Additional Formats
Product Specific Information
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing.
Acetone fixation is recommended for frozen sections.
Suggested positive control: human tonsil.
For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
Mouse anti-Human CD104 antibody, clone 450-9D recognizes the human beta4 integrin, also known as CD104.
Target Information
Integrins are heterodimers composed of alpha and beta subunits that form noncovalent transmembrane glycoprotein receptors.
Different alpha/beta combinations determine ligand-binding specificity.
Integrins mediate cell adhesion and signal transduction that regulate gene expression and cell growth.
The integrin beta 4 subunit is a receptor for laminins and associates with the alpha 6 subunit, playing a key role in invasive carcinoma biology.
Mutations are linked to epidermolysis bullosa with pyloric atresia.
Multiple alternatively spliced transcript variants exist for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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