Thermo Fisher Scientific CD45 Monoclonal Antibody (IBL-3/16)
마우스 CD45 단백질을 인식하는 Rat IgG1 모노클로날 항체로, IHC(F), ICC/IF, Flow Cytometry, IP에 사용 가능. 포름알데히드 고정에 민감하며 아세톤 고정 권장. 비인간 종과 반응하지 않음. 단백질 G 정제, 액상 형태, 연구용 전용.
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Thermo Fisher Scientific CD45 Monoclonal Antibody (IBL-3/16)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | IBL-3/16 |
| Immunogen | Purified B cells from murine lymph nodes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_928422 |
Product Specific Information
- This antibody does not react with human or rat.
- The epitope recognized by this antibody is sensitive to formaldehyde fixation and tissue processing. Acetone fixation is recommended for frozen sections.
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Rat anti-Mouse CD45 antibody, clone IBL-3/16, recognizes murine Receptor-type tyrosine-protein phosphatase C (CD45), also known as Leukocyte common antigen, T200, or Lymphocyte antigen 5 (Ly5).
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) expressed in all nucleated hematopoietic cells, accounting for about 10% of lymphocyte surface proteins. It is absent in non-hematopoietic tissues and cell lines.
CD45 plays a key role in lymphocyte development and antigen signaling by regulating Src-family kinases. Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic regions.
CD45RA, an isoform, shows restricted expression among lymphoid subtypes. Isoforms vary in membrane domain translocation and expression depending on cell type and physiological state.
CD45 regulates T- and B-cell receptor signaling, suppresses JAK kinases, and influences cytokine receptor signaling. It also modulates integrin-mediated signaling, apoptosis, and immune function regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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