Thermo Fisher Scientific SKA2 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Upon entry into mitosis, the cell’s microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for proper attachment of chromosomes to the spindle MTs.
A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, is required for stable KT–MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in loss of both proteins from the KT, without impacting overall KT structure.
Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in maintaining the metaphase plate and spindle checkpoint silencing.
SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.