Thermo Fisher Scientific CD3E Recombinant Rabbit Monoclonal Antibody (BL-298-5D12)
Thermo Fisher Scientific의 CD3E 재조합 토끼 단일클론 항체로, 인간 CD3ε 단백질을 인식합니다. Western blot, IHC, ICC/IF, Flow cytometry, IP에 적합하며, 고순도 액상 형태로 제공됩니다. 연구용으로 T세포 신호전달 및 면역학 연구에 활용됩니다.
- 카탈로그번호
- A700016x (2개 옵션)
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL per 1 × 10⁶ cells |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BL-298-5D12 |
| Immunogen | Region between residues 157–207 of human CD3ε (CD3-TCR Complex) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 50 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note:
- For ICC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
The CD3 subunit complex is crucial for transducing antigen-recognition signals into the cytoplasm of T cells and regulating the cell surface expression of the TCR complex. T cell activation via TCR involves the CD3γ, CD3δ, CD3ε, and CD3ζ subunits, which are structurally related members of the immunoglobulin superfamily encoded on human chromosome 11.
CD3 components possess long cytoplasmic tails that associate with signal transduction molecules via a double tyrosine-based motif. CD3 may play roles in TCR-induced growth arrest, cell survival, and proliferation. The CD3 antigen is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells. Altered T lymphocyte percentages may be observed in certain autoimmune diseases. Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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