Thermo Fisher Scientific CD4 Recombinant Rabbit Monoclonal Antibody (BL-155-1C11)
CD4 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, Flow cytometry, IP에 사용 가능. 인간 CD4에 반응하며 HEK293 세포에서 발현. 고순도 정제된 액상 형태로 4°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution / Usage |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL per 1 × 10⁶ cells |
| Immunoprecipitation (IP) | 20 µL per 1 mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BL-155-1C11 |
| Immunogen | Recombinant protein representing the extracellular domain of human CD4 (residues 26–397) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with Tris-EDTA (pH 9.0) is recommended for FFPE tissue sections.
Target Information
The CD4 antigen is involved in the recognition of MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed on T helper cells, but also found in monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression is detectable in thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut. CD4 plays a key role in T-cell activation through association with the T-cell receptor complex and the tyrosine kinase Lck. It may also contribute to neuronal damage in infectious and immune-mediated CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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