Thermo Fisher Scientific CD247/CD3Z Recombinant Rabbit Monoclonal Antibody (BL-336-1B2)
Thermo Fisher Scientific의 CD247/CD3Z 재조합 Rabbit 단클론 항체로, 인간 CD3 zeta 체인을 표적합니다. WB, IHC, Flow, IP 등 다양한 응용에 적합하며 HEK293 세포에서 발현된 정제 항체입니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Thermo Fisher Scientific CD247/CD3Z Recombinant Rabbit Monoclonal Antibody (BL-336-1B2)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Flow Cytometry (Flow) | 0.5 µL per 1 × 10^6 cells |
| Immunoprecipitation (IP) | 20 µL/1 mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BL-336-1B2 |
| Immunogen | Synthetic peptide representing a region between residue 150 and the C-terminus (residue 164) of human T-cell surface glycoprotein CD3 zeta chain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.10 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with Mouse, Sheep, and Pig.
Target Information
CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of CD3 subunits (gamma, delta, epsilon, and zeta), which are structurally related members of the immunoglobulin superfamily encoded by genes on human chromosome 11.
The CD3 components have long cytoplasmic tails that associate with signal transduction molecules via a double tyrosine-based motif. CD3 may play a role in TCR-induced growth arrest, cell survival, and proliferation. The CD3 antigen is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells. Decreased T lymphocyte percentages may occur in autoimmune diseases.
Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency due to CD3delta/CD3epsilon/CD3zeta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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