Thermo Fisher Scientific CD154 (CD40 Ligand) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per million cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant human CD40 Ligand/TNFSF5 Glu108-Leu261 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD154 (CD40 Ligand),uniProtId:P29965-1,ncbiNodeId:9606,antigenRange:108-261,antigenLength:261,antigenImageFileName:PA5-46964_CD154_CD40_Ligand_P29965-1_Goat.svg,antigenImageFileNamePDP:PA5-46964_CD154_CD40_Ligand_P29965-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609019

Product Specific Information

In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse CD40 Ligand is observed and less than 1% cross-reactivity with recombinant human (rh) CD27 Ligand and rhCD30 Ligand is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.