Thermo Fisher Scientific CPI-17 Polyclonal Antibody
CPI-17 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체. Western blot, IHC, ICC, Flow cytometry에 적합하며, Human, Mouse, Rat 시료 반응. 항원 친화 크로마토그래피로 정제된 동결건조 제품으로, 재구성 시 500 µg/mL 농도 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived human CPI17 alpha recombinant protein (Position: L30–Q126) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage conditions | −20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2746973 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphatase. It was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, dominantly expressed in smooth muscle. Phosphorylation at Threonine 38 by PKC or Rho-kinase enhances inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and may be involved in calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. Dual phosphorylation at Serine 12 and Threonine 38 by MYPT-associated kinase (M110 kinase) has also been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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