Thermo Fisher Scientific CCL16 Polyclonal Antibody, PeproTech
Human CCL16 단백질을 인식하는 염소 유래 폴리클로날 항체로, Western blot, ELISA, Immunostaining, In vitro assay 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| ELISA (ELISA) | 0.5–2.0 µg/mL | 1 publication |
| In vitro Assay (IV) | – | 2 publications |
| Immunostaining (IS) | – | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human LEC (CCL16) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929354 |
Product Specific Information
AA Sequence of recombinant protein:
QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ.
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human LEC (CCL16). Anti-Human LEC (CCL16)-specific antibody was purified by affinity chromatography employing an immobilized Human LEC (CCL16) matrix.
Sandwich ELISA:
To detect Human LEC (CCL16) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human LEC (CCL16) (500-P125GBt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human LEC (CCL16).
Western Blot:
To detect Human LEC (CCL16) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant Human LEC (CCL16) is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
The CCL16 gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are secreted proteins involved in immunoregulatory and inflammatory processes. CC cytokines are characterized by two adjacent cysteines. CCL16 exhibits chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Expression of this gene is upregulated by IL-10.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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