Thermo Fisher Scientific CCL16 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

In vitro Assay (IV)

-

View 2 publications 2 publications

Immunostaining (IS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Goat

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human LEC (CCL16) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CCL16,uniProtId:O15467-1,ncbiNodeId:9606,antigenRange:24-120,antigenLength:120,antigenImageFileName:500-P125G-100UG_CCL16_O15467-1_Goat.svg,antigenImageFileNamePDP:500-P125G-100UG_CCL16_O15467-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929354

Product Specific Information

AA Sequence of recombinant protein: QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ.

Preparation: Produced from sera of goats immunized with highly pure Recombinant Human LEC (CCL16). Anti-Human LEC (CCL16)-specific antibody was purified by affinity chromatography employing an immobilized Human LEC (CCL16) matrix.

Sandwich ELISA: To detect Human LEC (CCL16) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human LEC (CCL16) (500-P125GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human (CCL16) LEC.

Western Blot: To detect Human LEC (CCL16) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LEC (CCL16) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CCL16 gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. CCL16 displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. This cytokine also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. The expression of this gene is upregulated by IL-10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.