
Thermo Fisher Scientific DEFA1 Polyclonal Antibody, Biotin, PeproTech
Human NP-1 단백질 검출용 Biotin 결합 염소 유래 다클론 항체. ELISA 및 Western blot에 최적화된 고순도 항원 친화 정제 제품. 인간 DEFA1에 특이적이며 연구용으로만 사용 가능.
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Thermo Fisher Scientific DEFA1 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 3.4 kDa Recombinant Human NP-1 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929364 |
Product Specific Information
AA Sequence of recombinant protein:
ACYCRIPACI AGERRYGTCI YQGRLWAFCC
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human NP-1. Anti-Human NP-1-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human NP-1 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human NP-1 (500-P126G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human NP-1.
Western Blot:
To detect hNP-1 by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hNP-1 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Package:
500-P126GBT-1MG will be provided as 2 × 500 µg.
Target Information
Defensins are a family of microbicidal and cytotoxic peptides involved in host defense. They are abundant in neutrophil granules and also found in mucosal epithelia such as the intestine, respiratory tract, urinary tract, and vagina.
Members of the defensin family share high sequence similarity and a conserved cysteine motif.
The protein encoded by this gene, defensin alpha 1, is found in neutrophil granules and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8.
This gene differs from defensin alpha 3 by only one amino acid, and both are subject to copy number variation.
Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: 500-P126GBT-25UG_DEFA1_P59665-1_Goat.svg, 500-P126GBT-25UG_DEFA1_P59665-1_Goat_PDP.jpeg)
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