Thermo Fisher Scientific Perforin-1 (Pore Forming Protein) (Apoptosis Marker) Recombinant Rabbit Monoclonal Antibody (PRF1/7077R)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Assay-dependent

Immunohistochemistry (PFA fixed) (IHC (PFA))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Target Information

Perforin is a major cytolytic protein stored in cytoplasmic granules of cytotoxic lymphocytes. It plays a key role in immune defense against tumors and viral infections by forming transmembrane pores in target cells. The protein consists of 555 amino acids, including a 21-amino-acid signal peptide, and has a molecular weight of approximately 70–75 kDa. Perforin shares structural homology with complement component C9 and functions through a similar pore-forming mechanism. It is specifically expressed in killer cell lines, not in helper T lymphocytes or other tumor cells. Mutations in the perforin gene (PRF1) are associated with familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare autosomal recessive immune disorder. Alternative splicing generates multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.