Thermo Fisher Scientific COX4 Monoclonal Antibody (IB52C31H10), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL

-

Immunocytochemistry (ICC/IF)

1 µg/mL

-

ELISA (ELISA)

Assay-Dependent

-

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

IB52C31H10

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573024

Product Specific Information

Description: The IB52C31H10 monoclonal antibody recognizes human COX IV. COX IV, also known as cytochrome c oxidase (COX) or Complex IV, is the terminal complex of the electron transport chain. COX IV is encoded by nuclear DNA and localized to the inner mitochondrial membrane. COX IV functions in energy metabolism and shuttles electrons from cytochrome c to oxygen. Dysfunction in COX IV is associated with increased production of reactive oxygen species and cellular toxicity. Genetic defects in COX IV results in severe, often fatal metabolic disorders that primarily affect tissues with high energy demands such as heart, brain, and muscle.

Applications Reported: This IB52C31H10 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, ELISA, and immunocytochemistry.

Applications Tested: This IB52C31H10 antibody has been tested by immunocytochemistry of methanol-fixed human cells and can be used at less than or equal to 1 µg/mL. The IB52C31H10 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4 (COX4I1) is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.