Thermo Fisher Scientific COX4 Monoclonal Antibody (IB52C31H10), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Alexa Fluor 488
• Request custom conjugation

Product Specific Information

Description:
The IB52C31H10 monoclonal antibody recognizes human COX IV, also known as cytochrome c oxidase (COX) or Complex IV, the terminal complex of the electron transport chain. COX IV is encoded by nuclear DNA and localized to the inner mitochondrial membrane, functioning in energy metabolism by shuttling electrons from cytochrome c to oxygen. Dysfunction in COX IV is linked to increased reactive oxygen species and cellular toxicity. Genetic defects can cause severe metabolic disorders affecting high-energy tissues such as heart, brain, and muscle.

Applications Reported:
This antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemistry (frozen and paraffin-embedded sections), microscopy, ELISA, and immunocytochemistry.

Applications Tested:
Tested by immunocytochemistry on methanol-fixed human cells and immunohistochemistry on formalin-fixed paraffin-embedded tissue using low pH antigen retrieval, effective at ≤1 µg/mL. Optimal dilution should be determined experimentally.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It couples electron transfer from cytochrome c to oxygen, contributing to the proton electrochemical gradient across the inner mitochondrial membrane. The complex has 13 subunits encoded by mitochondrial and nuclear DNA. COX4 (COX4I1) is one of the nuclear-coded subunits and may play a role in complex regulation and assembly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.