Thermo Fisher Scientific ZO-1 Monoclonal Antibody (R26.4C), eBioscience

Applications and Tested Dilutions

Product Specifications

Related Formats

• eFluor 570
• Request custom conjugation

Product Specific Information

The monoclonal antibody R26.4C recognizes the tight junction protein 1, ZO-1 (zona occludens) of rat, mouse, canine, and porcine.
ZO-1 is a member of the MAGUK family of tight junction-associated scaffolding proteins containing PDZ, SH3, and GUK domains.
It is an integral component of the tight junction complex, forming a barrier to paracellular movement and maintaining epithelial polarity.
ZO-1 interacts with ZO-2, ZO-3, occludin, cingulin, claudins (1–8), actin, α-catenin, AF-6, ZONAB, Apg-2, and JAM-A–C, anchoring the actin cytoskeleton to tight junctions.
ZO-1 and ZO-2 are known to regulate cell cycle and proliferation, and their downregulation has been associated with cancer progression.

Applications Reported:
Western blotting, immunohistochemical staining (frozen tissues), microscopy, and immunocytochemistry.

Applications Tested:
Validated for ICC/IF (formaldehyde-fixed, permeabilized cells) and IHC (frozen mouse tissues). Recommended ≤10 µg/mL; optimal dilution should be determined experimentally.

Target Information

ZO-1 forms complexes with ZO-2 or ZO-3 and associates with claudin, occludin, and F-actin at tight junction strands, linking the actin cytoskeleton to the junction.
ZO-1 expression is reduced in several breast cancer cell lines.
ZO-2 and ZO-3 are ubiquitously expressed in epithelial tight junctions, while ZO-1 is also found in cardiac myocyte junctions.
ZO-1 may play a role in signal transduction at cell-cell junctions.
Two transcript variants encoding distinct isoforms have been identified.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.