Thermo Fisher Scientific ZO-1 Monoclonal Antibody (R26.4C), eBioscience
ZO-1 단백질을 인식하는 R26.4C 단클론 항체로, Tight junction 연구에 적합합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능하며, Rat/IgG2a, kappa 아이소타입입니다. 고순도(>90%)로 정제되어 있으며 4°C에서 보관합니다.
- 판매단위
- pk
카탈로그
4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific ZO-1 Monoclonal Antibody (R26.4C), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | 5 publications |
| Immunohistochemistry (PFA fixed) | - | 1 publication |
| Immunohistochemistry (Frozen) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1:100 | 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Mouse, Rat |
| Published Species | Human, Mouse, Xenopus |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | R26.4C |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865447 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Related Formats
Product Specific Information
The monoclonal antibody R26.4C recognizes the tight junction protein 1, ZO-1 (zona occludens) of rat, mouse, canine, and porcine.
ZO-1 is a member of the MAGUK family of tight junction-associated scaffolding proteins containing PDZ, SH3, and GUK domains.
It is an integral component of the tight junction complex, forming a barrier to paracellular movement and maintaining epithelial polarity.
ZO-1 interacts with ZO-2, ZO-3, occludin, cingulin, claudins (1–8), actin, α-catenin, AF-6, ZONAB, Apg-2, and JAM-A–C, anchoring the actin cytoskeleton to tight junctions.
ZO-1 and ZO-2 are known to regulate cell cycle and proliferation, and their downregulation has been associated with cancer progression.
Applications Reported:
Western blotting, immunohistochemical staining (frozen tissues), microscopy, and immunocytochemistry.
Applications Tested:
Validated for ICC/IF (formaldehyde-fixed, permeabilized cells) and IHC (frozen mouse tissues). Recommended ≤10 µg/mL; optimal dilution should be determined experimentally.
Target Information
ZO-1 forms complexes with ZO-2 or ZO-3 and associates with claudin, occludin, and F-actin at tight junction strands, linking the actin cytoskeleton to the junction.
ZO-1 expression is reduced in several breast cancer cell lines.
ZO-2 and ZO-3 are ubiquitously expressed in epithelial tight junctions, while ZO-1 is also found in cardiac myocyte junctions.
ZO-1 may play a role in signal transduction at cell-cell junctions.
Two transcript variants encoding distinct isoforms have been identified.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.