Thermo Fisher Scientific CD59 Monoclonal Antibody (8D2B8)
인간 CD59 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 적합합니다. 정제된 재조합 CD59(aa 31-111)를 면역원으로 사용하였으며, Protein G로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8D2B8 |
| Immunogen | Purified recombinant fragment of human CD59 (amino acids 31–111) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538518 |
Product Specific Information
MA5-17046 targets CD59 in indirect ELISA, FACS, ICC, IHC, IF, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human CD59 (amino acids 31–111) expressed in E. coli.
MA5-17046 detects CD59 with a predicted molecular weight of approximately 14.2 kDa.
Target Information
CD59 (Protectin) is a small (18–20 kDa) GPI-anchored, ubiquitously expressed inhibitor of the membrane attack complex (MAC).
It is a key regulator that protects autologous cells from the terminal effector mechanism of the complement cascade.
CD59 associates with the C5b-8 complex and prevents formation of the cytolytic pore within the plasma membrane.
It also acts as a low-affinity ligand of human CD2, contributing to T cell costimulation and lymphocyte signal transduction.
Mutations in CD59 cause CD59 deficiency, leading to hemolytic anemia, thrombosis, and cerebral infarction.
Multiple alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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