Thermo Fisher Scientific CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), Alexa Fluor 700, eBioscience
마우스 CD71(Transferrin Receptor)을 인식하는 Rat 모노클론 항체로, Alexa Fluor 700 형광체가 결합된 제품입니다. Flow Cytometry 분석에 적합하며, 세포 성장 및 철분 흡수 관련 연구에 활용됩니다. 4°C 보관, 동결 금지.
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Thermo Fisher Scientific CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 0.25 µg/test
- View 7 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | R17217 (RI7 217.1.4) |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2784764 |
Additional Formats Available
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 685
- NovaFluor Red 710
- NovaFluor Red 755
- NovaFluor Yellow 690
- NovaFluor Yellow 730
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The R17217 monoclonal antibody reacts with mouse CD71, a 170–180 kDa type II transmembrane protein. CD71 (Transferrin Receptor) forms homodimers on the cell surface and is essential for cellular growth. It is highly expressed in immature proliferating cells and upregulated upon antigen or mitogen stimulation of T and B cells. Expression levels vary among mouse strains.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤0.25 µg/test (100 µL final volume). Recommended titration for optimal performance.
Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and is excited with a red laser (633–647 nm). Recommended optical setup: 685 LP mirror and 710/20 band pass filter.
Excitation: 633–647 nm
Emission: 723 nm
Laser: Red Laser
Target Information
Iron uptake occurs through receptor-mediated endocytosis of transferrin bound to its receptor. Endosomal acidification releases iron, and the apotransferrin-receptor complex is recycled back to the surface. Transferrin receptor is vital for erythrocyte and nervous system development. Serum transferrin receptor (sTfR) serves as a marker for EPO misuse detection and anemia diagnostics in conditions such as rheumatoid arthritis, pregnancy, IBS, and HIV.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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