Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f (integrin alpha 6), also known as VLA-6 alpha chain and platelet gpIc. CD49f is a 120 kDa transmembrane protein that associates with CD29 (integrin beta 1) to form VLA-6, or with CD104 (integrin beta 4) to form alpha6beta4 complexes. It is expressed on T cells, monocytes, platelets, epithelial and endothelial cells, and germinal center B cells. The antibody cross-reacts with human, mouse, and bovine integrin alpha 6 and can block its binding to laminin.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. May be used at ≤0.25 µg/test (defined as 100 µL reaction volume). Recommended to titrate for optimal performance.

Fluorochrome Characteristics:
Alexa Fluor 700 emits at 723 nm and is excited with a red laser (633–647 nm). Instruments typically require a 685 LP mirror and 710/20 band pass filter.
Excitation: 633–647 nm
Emission: 723 nm
Laser: Red

Target Information

CD49f (Integrin alpha-6, ITGA6):
Integrin alpha 6 is an alpha chain subunit that pairs with beta chains (e.g., beta 1 or beta 4) forming distinct integrins such as VLA-6 or TSP180. Integrins mediate cell adhesion and signal transduction between the extracellular matrix (ECM) and cytoskeleton, influencing cell shape, motility, and proliferation. CD49f is mainly expressed in epithelial cells and plays a key structural role in hemidesmosomes. Multiple alpha and beta subunit isoforms exist in mammals, enabling diverse cell adhesion and signaling functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지