Thermo Fisher Scientific SEMA4D Monoclonal Antibody (OTI8E6), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:500

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Semaphorins are neuronal chemorepellents that guide pioneering neurons during nervous system development.
CD100 (also known as Semaphorin 4D) is a 150 kDa homodimer that induces B cell aggregation and enhances their viability in vitro.
It modulates CD40-CD40L B cell signaling by increasing B cell aggregation and survival while downregulating CD23 expression.
CD72 acts as a lymphocyte receptor for the class IV semaphorin CD100 and plays a role in regulating B cell signaling.
CD100 is expressed on resting and PHA-stimulated T cells, weakly on NK cells, EBV-transformed B cells, monocytes, and tumor/peripheral blood T cell lines, and at higher levels on activated T and B cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.