Thermo Fisher Scientific Human IFN-alpha A/D Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Molecular weight

19.4 kDa

Class

Recombinant

Type

Protein

Purity

>95%

Activity

Specific Activity = 1.8x10e8 units/mg; Assay used to measure bioactivity: Interferon was titrated with the use of the cytopathic effect inhibition assay as described by Rubinstein, et al. In this antiviral assay for interferon about 1 unit/mL of interferon is the quantity necessary to produce a 50% cytopathic effect. The units are determined with respect to the international reference standard for human interferon alpha A (Hu-IFN-αA) provided by the National Institutes of Health. The units of activity are determined with bovine MDBK cells infected with vesicular stomatitis virus (VSV).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS with 0.1% BSA

Contains

no preservative

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Carrier-Free

Dilution Recommendation: Further dilutions should be made in medium or buffered solution containing carrier protein such as PBS with 0.1% BSA.

Target Information

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.