Thermo Fisher Scientific Human IFN-beta 1a Recombinant Protein
인간 IFN-beta 1a 재조합 단백질로, CHO 세포에서 발현된 고순도(>95%) 액상 형태 시약입니다. 항바이러스 및 항종양 연구용으로 WB, IHC, ELISA 등 다양한 분석에 활용 가능하며, -80°C에서 보관합니다.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | CHO cells |
| Molecular Weight | 23 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% |
| Endotoxin Concentration | <1 EU/µg |
| Activity | EC50 ~ 1 U/mL; determined by a cytopathic inhibition assay on human A549 lung carcinoma cells with encephalomyocarditis virus (EMCV) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Purified |
| Storage Buffer | 50 mM sodium acetate with 0.1% BSA |
| Contains | No preservative |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry ice |
Product Specific Information
- Source: cDNA obtained from human fibroblast interferon beta 1a mRNA expressed in CHO cells.
- Storage:
- After receipt, store at -20°C for short-term use (<6 months).
- For long-term storage, store at -70°C or below for full activity retention.
- Thaw vial in cold tap water until just thawed.
- Aliquot contents to minimize freeze-thaw cycles.
- Refreeze on dry ice or in a dry ice/alcohol bath.
- Further dilution should be in protein-containing buffers (e.g., 0.1% BSA).
Target Information
Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit tumor development. IFN-beta binding activates tyrosine kinases Jak1 and Tyk2, leading to phosphorylation of STAT transcription factors and expression of immune response genes. Members of the toll-like receptor (TLR) family also stimulate IFN-beta production. Clinically, IFN-beta is used for treatment of tumors, infections, and multiple sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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