Thermo Fisher Scientific Human IFN-beta 1a Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

CHO cells

Molecular weight

23 kDa

Class

Recombinant

Type

Protein

Purity

>95%

Endotoxin concentration

<1 EU/µg

Activity

EC50 ~ 1 U/mL; determined by a cytopathic inhibition assay on human A549 lung carcinoma cells with encephalomyocarditis virus (EMCV).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

50mM sodium acetate with 0.1% BSA

Contains

no preservative

Storage conditions

-80°C

Shipping conditions

Dry ice

Product Specific Information

Source: cDNA obtained from human fibroblast interferon beta 1a mRNA expressed in CHO cells.

Storage: After receipt, the product may be stored at -20ºC for short-term use (<6 months). For long-term storage, store the product at -70ºC or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. The contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Target Information

Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.