
Thermo Fisher Scientific Human GM-CSF, AOF Recombinant Protein
인간 GM-CSF 재조합 단백질로, 순도 98% 이상 및 낮은 엔도톡신 수준을 특징으로 함. 세포 증식 기능 분석, ELISA, 웨스턴 블롯 등 다양한 연구 응용에 적합. 동결건조 형태로 안정적이며 장기 보관 가능.
- 카탈로그번호
- PHC6025
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
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Thermo Fisher Scientific Human GM-CSF, AOF Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Human GM-CSF recombinant protein contains 128 amino acids |
| Molecular Weight | 14.6 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >98% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <1 EU/µg |
| Activity | ED50 < 0.1 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
Briefly centrifuge vial before opening to bring contents to the bottom. Reconstitute GM-CSF in sterile, distilled water to a concentration of 0.1–1.0 mg/mL. Further dilutions should be made in aqueous buffers supplemented with carrier protein.
Storage:
Lyophilized preparation is stable at 2–8°C but should be kept at -20°C for long-term storage, preferably desiccated. Upon reconstitution, stable for up to one week at 2–8°C. For maximal stability, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
The GM-CSF gene encodes a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active protein form exists as a homodimer in the extracellular space.
This gene is located within a cluster on chromosome 5q31, associated with the 5q- syndrome and acute myelogenous leukemia. Related genes in the cluster include interleukins 4, 5, and 13.
GM-CSF promotes tissue inflammation, and elevated levels have been reported in SARS-CoV-2 patients with acute respiratory distress syndrome. Mice lacking this gene or its receptor develop pulmonary alveolar proteinosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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