Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI5B10), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (proliferating cell nuclear antigen) is essential for DNA replication and participates in DNA excision and mismatch repair pathways.
It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and is useful for assessing tumor proliferation status.
It is localized in the nucleus, acts as a cofactor of DNA polymerase delta, and forms a homotrimer that enhances leading strand synthesis during replication.
In response to DNA damage, PCNA is ubiquitinated and functions in the RAD6-dependent DNA repair pathway.
Two transcript variants encoding the same protein have been identified, with pseudogenes located on chromosome 4 and the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.