Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI4A2), TrueMAB

Applications and Tested Dilution

• Western Blot (WB): 1:2,000

Product Specifications

Additional Formats

• Biotin Conjugate
• Request custom conjugation

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL. For conjugation experiments using this carrier-free antibody, an additional desalting step is recommended (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (Proliferating Cell Nuclear Antigen) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA serves as a marker for proliferating cells and tumor prognosis, being expressed in early G1 and S phases. It functions as a cofactor of DNA polymerase delta, forming a homotrimer to enhance DNA synthesis processivity. Upon DNA damage, PCNA undergoes ubiquitination and participates in the RAD6-dependent repair pathway. Two transcript variants encoding the same protein have been identified, with pseudogenes located on chromosome 4 and the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.