Thermo Fisher Scientific SIT Monoclonal Antibody (SIT-01)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Flow Cytometry (Flow)

1-5 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

SIT-01

Immunogen

Bacterially produced, recombinant, intracellular fragment of human SIT.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• PE
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2301877

Product Specific Information

This antibody reacts with murine SIT.

Western Blot: Reducing conditions.

Target Information

SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.