Thermo Fisher Scientific SIT Monoclonal Antibody (SIT-01)
SIT 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot, Flow cytometry, Immunoprecipitation에 사용 가능. 인간 시료에 반응하며, Protein A로 정제된 액상 형태. 4°C 보관, 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–5 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SIT-01 |
| Immunogen | Bacterially produced, recombinant, intracellular fragment of human SIT |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2301877 |
Additional Formats
Product Specific Information
This antibody reacts with murine SIT.
Western Blot: Reducing conditions.
Target Information
SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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