Thermo Fisher Scientific NUMB Monoclonal Antibody (OTI5C5), TrueMAB
인간 NUMB 단백질(1–365 aa)을 항원으로 제작된 Mouse IgG1 단클론 항체. Western blot에 1:2,000 희석으로 사용 가능. 동결건조 형태로 제공되며 PBS와 trehalose로 안정화. 신경세포 분화 및 신경돌기 성장 연구에 적합.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–365 of human NUMB produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Protein NUMB homolog is an evolutionarily conserved protein that regulates neuronal progenitor cell differentiation. It participates in clathrin-dependent endocytosis via interaction with the AP-2 complex and is expressed in postmitotic neurons, contributing to axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domains exhibit extensive neurite outgrowth, which can be inhibited by VGCC blockers or Ca²⁺ chelators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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