Thermo Fisher Scientific NUMB Monoclonal Antibody (OTI3E5), TrueMAB
Human NUMB 단백질을 인식하는 Mouse monoclonal antibody로 Western blot 및 IHC(Paraffin)에 적합. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도. PBS buffer(8% trehalose) 기반, 보존제 무첨가. -20°C 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3E5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–365 of human NUMB produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Protein NUMB homolog is an evolutionarily conserved protein that regulates the differentiation of neuronal progenitor cells.
NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex, and is expressed in postmitotic neurons where it contributes to axonal growth.
Neural cells expressing Numb isoforms with a short phosphotyrosine-binding (SPTB) domain exhibit extensive neurite outgrowth, which can be inhibited by voltage-gated Ca²⁺ channel (VGCC) inhibitors or Ca²⁺ chelators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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