Thermo Fisher Scientific CaM Kinase IV Polyclonal Antibody
CaM Kinase IV에 특이적인 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot 및 IHC에 적합하며, Human과 Rat 시료에서 반응. 항원 친화 크로마토그래피로 정제된 고순도 제품으로, 세포 내 칼슘 신호 및 전사 조절 연구에 활용 가능.
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Thermo Fisher Scientific CaM Kinase IV Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 2–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from aa 457–474 of rat CaM Kinase IV |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Jurkat cells, Brain
- Cellular location: Cytoplasmic and Nuclear
Target Information
CAMK4 (CaMKIV) is a serine/threonine protein kinase that phosphorylates CREB, oncoprotein 18, and SRF. It has limited tissue distribution and is implicated in transcriptional regulation in lymphocytes, neurons, and male germ cells.
The nuclear localization of CaMKIV aligns with its role in calcium-dependent gene expression. It is abundant in testis, T-cells, and neurons, and also found in other tissues.
In neurons, CaMKIV contributes to synaptic plasticity via gene regulatory mechanisms, while in T-cells it plays a role in calcium signaling affecting NFAT transcriptional regulation.
CaMKIV is encoded along with calspermin by the CaMKIV gene and is associated with chromatin in germ cells, suggesting a role in chromatin remodeling during spermatid nuclear condensation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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