Thermo Fisher Scientific L1CAM Monoclonal Antibody (OTI4F4), TrueMAB
L1CAM 단백질을 인식하는 마우스 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry, IP에 적합합니다. 인간 시료에 반응하며, 동결건조 형태로 제공되어 안정적 보관이 가능합니다. 신경계 발달 연구 및 CD171 관련 연구에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2–4 µg/mg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F4 |
| Immunogen | Full-length human recombinant protein of L1CAM (CD171, UniProt ID: P32004-1, 1–1257 aa) produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
L1CAM (CD171) is an axonal glycoprotein belonging to the immunoglobulin superfamily. It contains multiple immunoglobulin-like and fibronectin type III domains, linked by a single transmembrane segment to a conserved cytoplasmic domain. L1CAM plays a critical role in nervous system development, including neuronal migration and differentiation. Mutations in this gene are associated with CRASH syndrome (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia, and hydrocephalus). Alternative splicing of a neuron-specific exon may have functional significance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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