Thermo Fisher Scientific Phospho-p53 (Thr55) Recombinant Rabbit Monoclonal Antibody (ST0439)

Thermo Fisher Scientific Phospho-p53 (Thr55) Recombinant Rabbit Monoclonal Antibody (ST0439)

Applications and Tested Dilution

• Western Blot (WB): 1:1,000–1:2,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:400

Product Specifications

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:

• Improved specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation.
Key features:

• Up-regulated by stress signals to activate transcription of genes such as p21waf1
• Contains N-terminal transactivation, central DNA-binding, oligomerisation, and C-terminal regulatory domains
• Phosphorylation at Ser15 is critical for activation and stabilization
• Plays a role in preventing proliferation of damaged cells and acts as an anticancer agent
• Alterations or mutations in TP53 are linked to various human cancers and syndromes such as Li-Fraumeni

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.