Thermo Fisher Scientific Phospho-p53 (Ser376) Recombinant Rabbit Monoclonal Antibody (ST0440)

Applications

• Western Blot (WB): 1:1,000 dilution

Product Specifications

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages include:

• High specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broad immunoreactivity due to large rabbit immune repertoire

Target Information

The tumor suppressor protein p53 is a sequence-specific transcription factor activated by cellular stress. It mediates cell cycle arrest or apoptosis in response to DNA damage or nutrient deprivation.
Key characteristics:

• Upregulated under stress to activate transcription of genes such as p21waf1
• Contains N-terminal transactivation, central DNA-binding, oligomerization, and C-terminal regulatory domains
• Phosphorylation at Ser15 is crucial for activation and stabilization
• Phosphorylation at Ser392 is associated with tumor formation
• Plays roles in aging, oxidative stress, learning, and memory
• Highly expressed in transformed cells, contributing to malignancy
• Mutations in TP53 gene lead to loss of tumor suppressor activity and are linked to various cancers, including Li-Fraumeni syndrome

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.