Thermo Fisher Scientific CD18 Recombinant Mouse Monoclonal Antibody (1B4)
CD18 단백질을 인식하는 마우스 유래 재조합 단클론 항체로, 인간 및 붉은털원숭이 시료에 반응합니다. ICC, Flow, ELISA, IP, RIA 등 다양한 면역실험에 사용 가능하며, Protein A로 정제된 액상 형태입니다. 단기 4°C, 장기 -20°C 보관 권장.
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1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD18 Recombinant Mouse Monoclonal Antibody (1B4)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Radioimmune Assays (RIA) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG2a, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1B4 |
| Immunogen | A BALB/c mouse was injected with 10^6 monocytes from 4 healthy donors three times at monthly intervals, and four times with 5x10^6 monocytes on each of the four days before fusion. The spleen cells were then fused to P3U1 myeloma cells to generate hybridomas and RIA was used to identify lines of interest. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
Product Specific Information
Specific to the beta chain of the leukocyte integrin beta-2 family. This is found in LFA-1, Mac-1 and p150.95 among others. Binds an epitope distinct from the C3bi binding site. Has been shown to recognise both the human and Rhesus monkey antigen.
Target Information
CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes.
CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling.
CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins:
- αLβ2 (CD11a/CD18, LFA-1)
- αMβ2 (CD11b/CD18, Mac-1, CR3)
- αXβ2 (CD11c/CD18)
- αDβ2 (CD11d/CD18)
These integrins are essential for proper leukocyte migration and intercellular contact mediation.
Absence of CD18 leads to leukocyte adhesion deficiency-1 (LAD1), and reduced expression causes psoriasiform skin disease.
CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin, mediating cytolysis.
Two transcript variants encoding the CD18 protein have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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