Thermo Fisher Scientific Phospho-SMAD2 (Ser465, Ser467) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:3,000

ELISA

• Tested Dilution: 1:10,000

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot contents and freeze at -20°C or below for extended storage.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

Anti-SMAD2 pS465 pS467 antibody is directed against the phosphorylated form of human SMAD2 protein at the pS465 and pS467 residues. Reactivity occurs against human SMAD2. Reactivity with non-phosphorylated SMAD2 is minimal by ELISA and Western blot.
A BLAST analysis suggests cross-reactivity with SMAD2 from human, mouse, rat, orangutan, and bovine based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Target Information

SMAD2 (MADH2, MAD2) regulates multiple cellular processes such as cell proliferation, apoptosis, and differentiation. It interacts with TGF-beta receptors through the SMAD anchor for receptor activation, playing a central role in TGF-beta signaling.
Phosphorylation of SMAD2 by TGF-beta receptors induces dissociation from SARA and association with SMAD4, facilitating nuclear translocation and transcriptional regulation. SMAD2 can also be phosphorylated by activin type 1 receptor kinase, mediating activin signaling.
Alternative splicing variants encoding SMAD2 have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.