Thermo Fisher Scientific Gata1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:3,000

-

ELISA (ELISA)

1:15,000-1:75,000

-

Immunoprecipitation (IP)

1:500-1:2,000

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Gata1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to mouse Gata1 peptide.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.73 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Gata1 is directed against mouse Gata1. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence and 93% to human. Cross reactivity with Gata1 homologues from other sources has not been determined.

Target Information

Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5-\[AT\]GATA\[AG\]-3 within regulatory regions of globin genes and of other genes expressed in erythroid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.