Thermo Fisher Scientific Serpina12 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:

MLKPSFSPRN YKALSEVQGW KQRMAAKELA RQNMDLGFKL LKKLAFYNPG RNIFLSPLSI STAFSMLCLG AQDSTLDEIK QGFNFRKMPE KDLHEGFHYI IHELTQKTQD LKLSIGNTLF IDQRLQPQRK FLEDAKNFYS AETILTNFQN LEMAQKQIND FISQKTHGKI NNLIENIDPG TVMLLANYIF FRARWKHEFD PNVTKEEDFF LEKNSSVKVP MMFRSGIYQV GYDDKLSCTI LEIPYQKNIT AIFILPDEGK LKHLEKGLQV DTFSRWKTLL SRRVVDVSVP RLHMTGTFDL KKTLSYIGVS KIFEEHGDLT KIAPHRSLKV GEAVHKAELK MDERGTEGAA GTGAQTLPME TPLVVKIDKP YLLLIYSEKI PSVLFLGKIV NPIGK

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Vaspin. Anti-Human Vaspin-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hVaspin by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Vaspin (500-P256) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hVaspin.

Western Blot:
To detect hVaspin by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant hVaspin is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package:
500-P256BT-1MG will be provided as 2 × 500 µg.

Target Information

Vaspin is a newly described adipocytokine expressed predominantly in visceral white adipose tissues. Structure analysis of Vaspin predicts the presence of three beta-sheets, nine alpha-helices, and one central loop, which are distinctive structural features of Serpin family members.
The serpins are irreversible (“suicidal”) serine-protease inhibitors, characterized by more than 30% sequence homology with alpha1-antitrypsin and a conserved tertiary structure containing an exposed reactive center loop that acts as a pseudo-substrate for the target proteinase.

Members of this family play important roles in fundamental biological processes, including blood coagulation, fibrinolysis, complement activation, angiogenesis, inflammation, and tumor suppression.
In humans, serpins represent approximately 2% of total serum proteins, of which 70% is alpha1-antitrypsin. Vaspin exhibits 40.2% sequence identity with alpha1-antitrypsin, though its protease inhibitory activity remains unknown.

Vaspin mRNA expression in visceral fat is positively correlated with BMI and percent body fat. Administration of Vaspin to obese mice improved glucose tolerance and insulin sensitivity, normalized blood glucose levels, and reversed altered expression of diabetes-related adipocytokines such as leptin, adiponectin, resistin, and TNF-alpha.
These findings suggest potential clinical use for Vaspin in ameliorating certain metabolic abnormalities associated with diabetes and obesity.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.