Thermo Fisher Scientific CTAGE5 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein
MLESTKLLAD LKKCGDLECE ALINRVSAMR DYRGPDCRYL NFTKGEEISV YVKLAGERED LWAGSKGKEF GYFPRDAVQI EEVFISEEIQ MSTKESDFLC L

Preparation
Produced from sera of rabbits immunized with highly pure Recombinant Human MIA-2. Anti-Human MIA-2-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA
To detect Human MIA-2 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MIA-2 (500-P255) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human MIA-2.

Western Blot
To detect Human MIA-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 mg/mL. When used with compatible development reagents, the detection limit for Recombinant Human MIA-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging
500-P255BT-1MG will be provided as 2 × 500 µg.

Target Information

CTAGE5/MIA-2 is a secreted cytokine and a member of the MIA/OTOR family, which also includes MIA, OTOR, and TANGO. These proteins share a Src homology-3 (SH3)-like domain. MIA-2 is predominantly expressed in hepatocytes, and elevated levels may serve as a clinically useful marker for hepatic disease activity and severity.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.